EFFECTIVENESS OF LACTIC ACID BACTERIA SOLUTION CHALLENGE TEST BY USING DIFFERENT ENVIRONMENTAL CONTROLS CAVENDISH BANANA PEEL FERMENTATION RESULTS AGAINST MACKEREL FISH REVERSE BACTERIA
Authors
Reza Salsabila , Evi Liviawaty , Junianto Junianto , Rusky Intan PratamaDOI:
10.29303/jp.v14i2.859Published:
2024-06-26Issue:
Vol. 14 No. 2 (2024): JURNAL PERIKANANKeywords:
Environmental Control, Challenge Test, Clear Zone, Spoilage BacteriaArticles
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Abstract
Mackerel has an economical price, high market demand, and high catch yields so that it is widely used by the wider community compared to other types of pelagic fish, but it has disadvantages, namely that it is highly perishable because after being caught, the fish still undergoes a series of change processes before becoming rotten. Lactic acid bacteria can be applied to preserve fishery products by immersing them in a culture of lactic acid bacteria derived from fermentation. Cavendish banana peel fermentation contains secondary metabolite compounds that have the potential to be antimicrobial, anticancer and antidemartosis. Cavendish banana peels contain flavonoids, alkaloids, tannins, saponins and quinones. This research aims to determine the concentration of environmental controls between salt and vinegar in the Lactic Acid Bacteria fermentation solution from Cavendish banana peels which can provide effectiveness. lactic acid bacteria against mackerel spoilage bacteria. This research was carried out at the Fishery Products Processing Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University in January 2024. This research was carried out by testing Lactic Acid Bacteria from the fermented solution of Cavendish banana peels and spoilage bacteria from mackerel that was soaked for 15 minutes. using paper discs with 3 treatments and 9 repetitions. Observations were made at the 48th hour after incubation at a storage temperature in an incubator of 37oC covering the diameter of the clear zone around the paper disc. The calculation results for estimating the addition of the best environmental control concentration were carried out by analyzing the data using the simultaneous F test followed by the Duncan test. The research results show that the best environmental control concentration addition for Cavendish banana peel fermentation as a source of Lactic Acid Bacteria against mackerel spoilage bacteria to extend shelf life is the addition of 3% salt with a pH of 4.4, a clear zone diameter of 21.37 mm and an effectiveness of 34.1% which is categorized as very strong.
References
Abdel-Rahman, S. G., Desouky, S. G., & Nithya, K. (2013). Effect of antimicrobial metabolites produced by lactic acid bacteria on quality aspects of frozen tilapia (Oreochromis niloticus) fillets. Journal of Fish and Marine Sciences, 1(1), 40-45.
Adam, A. A., Posangi, J., Tumewu, E., & Tallei, T. E. (2014). Aktivitas antibakteri ekstrak kasar tunikata Polycarpa aurata terhadap Streptococcus mutans. Dentire Journal, 3(2), 118-122.
Adhayanti, Abdullah, I., & Romantika, R. (2018). Uji kandungan total polifenol dan flavonoid ekstrak etil asetat kulit pisang raja (Musa paradisiaca). Media Farm, 14(1), 1-7.
Atun, Bayu, E. S., & Setiado, H. (2007). Identifikasi karakter morfologi pisang (Musa sp.) di Kabupaten Deli Serdang. Jurnal Agroekoteknologi, 4(1), 1911-1924.
Batubara, R. W., Suherman, A., & Mudzakir, K. (2022). Pola musim penangkapan ikan kembung yang didaratkan di Pelabuhan Perikanan Pantai Asemdoyong Pemalang. Jurnal Penelitian Perikanan Indonesia, 27(4), 203-215.
Chang, J. Y., & Chang, H. C. (2011). Improvements in the quality and shelf life of kimchi by fermentation with the induced bacteriocin-producing strain, Leuconostoc citreum GJ7 as a starter. Journal of Food Science, 75(2), 103-110.
Cowan, M. M. (1999). Plants product as antimicrobial agents. Clinical Microbiology Reviews, 564-582.
Cushnie, A. (2005). Uji Efektifitas Ekstrak Madu Karet dalam Menghambat Pertumbuhan Staphylococcus aureus (Skripsi Sarjana, Universitas Islam Negeri Syarif Hidayatullah).
Daulay, D., & Rahman, A. (1992). Teknologi Fermentasi Sayur-Sayuran dan Buah-Buahan. Bogor: IPB.
Davis, K., & Stout, G. R. (1971). Characterization of bacteriocin producing lactic acid bacteria and its application as a food preservative. African Journal of Microbiology Research, 6(6), 1138-1146.
Eldo, D., & Theophilus, W. W. (2015). Uji daya hambat ekstrak etanol suruhan (Piperomia pellucida) terhadap pertumbuhan bakteri Staphylococcus aureus secara in vitro. Jurnal Mikrobiologi, 2(1), 1-14.
Fibriarti, Wikandari, & Samsu, W. (2018). Efektivitas bakteri asam laktat terhadap masa simpan dan mutu ikan kakap (Lutjanus campechanus). Jurnal Ilmiah Peternakan, 1(1), 151-156.
Gani, S., Margino, S., Triyanto, & Setyati, W. A. (2018). Effects of pH, temperature and salinity in growth and organic acid production of lactic acid bacteria isolated from penaeid shrimp intestine. Indonesian Journal of Marine Sciences, 2(1), 18-30.
Gao, X., Jiang, L., Hu, Y., & Hou, L. (2020). Isolasi dan karakterisasi bakteri asam laktat dari limbah kotoran ayam sebagai agensi probiotik dan enzim kolesterol reduktase. Biologi dan Pengembangan Profesi Pendidik Biologi, Biota, 12(3), 177-185.
Gonzales, Haryadi, Djagal, W. M., & Cahyanto, M. N. (1996). Isolasi dan karakterisasi bakteri asam laktat amilolitik selama fermentasi growol, makanan tradisional Indonesia. Jurnal Teknologi Pertanian, 13(1), 52-60.
Hong, Y., Yang, H. S., & Chang, H. C. (2021). Comparison of bacterial community changes in fermenting kimchi at two different temperatures using a denaturing gradient gel electrophoresis analysis. Journal of Microbiology and Biotechnology, 23(1), 76-84.
Hutkins, T. (2006). Isolation of lactic acid bacteria from cabbage and their potential inhibition to pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella typhimurium). Jurnal Teknologi Pertanian, 10(1), 19-27.
Khoiriyah, H., & Ardiningsih, P. (2014). Penentuan waktu inkubasi optimum terhadap aktivitas bakteriosin Lactobacillus sp. Jurnal Kimia Khatulistiwa, 3(4), 21-31.
Lee, M. A., Choi, Y. J., Kim, Y. S., Chon, S. Y., Chung, Y. B., Park, S. H., Yun, Y. R., Min, S. G., Yang, H. C., & Seo, H. Y. (2022). Effects of salt type on the metabolites and microbial community in kimchi fermentation. Heliyon, 8(11), 11-36.
Mahatmanti, A., Siti, H., & Dadan, R. (2007). Aktivitas antibakteri ekstrak teh putih terhadap bakteri gram positif dan negatif. Jurnal Penelitian Teh dan Kina, 18(1), 55-60.
Mappiratu, & Bakhri, S. (2013). Penuntuk Praktikum Bioteknologi. Palu: Jurusan Kimia Fakultas MIPA Universitas Tadulako.
Meiliawati. (2017). Fase pertumbuhan isolat bakteri asam laktat (BAL) tempoyak asal Jambi yang disimpan pada suhu kamar. Jurnal Sain Peternakan Indonesia, 11(1), 58-66.
Muafi, M. (2004). Uji efektivitas ekstrak daun pepaya (Carica papaya) terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Kesehatan, 7(3), 21-33.
Naim, G. (2004). Aktivitas antibakteri ekstrak herbal krokot (Portulaca oleracea) terhadap Staphylococcus aureus dan Escherichia coli. Lentera Bio, 2(1), 87-93.
Putri, A. Y. (2021). Uji Aktivitas dan Efektivitas Antibakteri Ekstrak dan Fraksinasi.
Ratnakomala, T., Marwati, & Ardhani, F. (2006). Uji daya hambat bakteri Staphylococcus aureus menggunakan ekstrak daun tahongai (Kleinhovia hospita). Jurnal Peternakan Lingkungan Tropis, 2(2), 41-50.
Rijayanti, R. P. (2014). Uji aktivitas antibakteri ekstrak etanol daun mangga bacang (Mangifera foetida) terhadap Staphylococcus aureus secara in vitro. (Skripsi Sarjana, Universitas Tanjungpura).
Riyanto, S., & Mardiansjah, F. H. (2018). Kajian pengembangan industri pengolahan perikanan dalam pengembangan ekonomi lokal di Kabupaten Pati. Jurnal Pembangunan Wilayah dan Kota, 14(1), 61-71.
Rubai, H. H., Hassan, K. H. A., & Eskandder, Z. E. (2020). Drying and salting fish using different methods and their effect on the sensory, chemical, and microbial indices. Multidisciplinary, 3(1), 10-17.
Rujiah, & Apriyanto. (2017). Uji antibakteri ekstrak etanol daun jarak pagar (Jatropha cuircas L) terhadap bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408. Jurnal Ilmu-Ilmu Pertanian, 2(1), 26-37.
Sabir, K. (2005). Fermentative utilization of fruit peel waste for lactic acid production by Lactobacillus plantarum. Indian Journal of Applied Research, 4(9), 449-451.
Safrida, A., Siti, H., & Dadan, R. (2012). Aktivitas antibakteri ekstrak teh putih terhadap bakteri gram positif dan negatif. Jurnal Penelitian Teh dan Kina, 18(1), 55-60.
Saraswati, I. (2005). Berkebun Pisang Cavendish Secara Komersial. Jakarta: Penebar Swadaya.
Sartika, Widyastuti, Y., & Sofarianawati, E. (2020). Karakter bakteri asam laktat Enterococcus sp. yang diisolasi dari saluran pencernaan ternak. Jurnal Mikrobiologi Indonesia, 4(2), 50-53.
Sawitri, D. P., Melkior, P., Sefy, A. I., Markus, B. R., & Wasilah. (2013). Lactobacillus acidophilus probiotic inhibits the growth of Candida albicans. J Dent Indonesia, 20(3), 64-67.
Sukasih, F. M. (2018). Penelusuran pustaka kandungan senyawa dari ekstrak kulit pisang raja (Musa paradisiaca var raja) dan kulit pisang Cavendish (Musa cavendishii) dalam beberapa aktivitas farmakologi. Jurnal Bandung Conference Series: Pharmacy, 2(1), 1-12.
Sulistijowati, R. (2014). Identification of lactic acid bacteria isolated from milkfish intestine (Chanos chanos). In Proceeding International Seminar Innovation on Marine and Fisheries Product Processing and Biotechnology Towards the ASEAN Economic Community in 2015. Jakarta.
Supriati, G., Pelealu, J. J., & Trina, E. (2015). Isolasi dan identifikasi bakteri asam laktat hasil fermentasi kol merah (Brassica oleracea) sebagai probiotik potensial. Jurnal Bioslogos, 8(2), 1-10.
Supriati, R., Ekowati, C. N., & Sinaga, A. (2015). Pengaruh pH terhadap produksi antibakteri oleh bakteri asam laktat dari usus itik. Jurnal Penelitian Pertanian Terapan, 15(3), 234-238.
Suprihatin. (2010). Teknologi Fermentasi. Surabaya: UNESA University Press.
Tallei, T. E., Fatimawali, & Sumual, A. M. (2019). Uji antibakteri dari bakteri asam laktat hasil fermentasi selada romain (Lactuca sativa var). Pharmacon Journal, 8(2), 306-314.
Tortora, M. F., Rohula, U., & Esti, W. (1998). Kajian karakteristik minuman sinbiotik pisang kepok (Musa paradisiaca forma typical) dengan menggunakan starter Lactobacillus acidophilus IFO 13951 dan Bifidobacterium longum ATCC 15707. Jurnal Teknosains Pangan, 1(1), 1-11.
Utami, T. (2013). Aktivitas Antibakteri Ekstrak Etanol Bunga Rosella (Hibiscus sabdariffa) Terhadap Escherichia coli, Salmonella typhi dan Staphylococcus aureus dengan Metode Difusi Agar. (Skripsi Sarjana, Universitas Padjadjaran).
Waluyo, M. (1984). Pengaruh Sediaan Teh (Camellia sinensis) dan Madu Terhadap Bakteri Salmonella typhi dan Staphylococcus aureus. (Skripsi Sarjana, Universitas Andalas).
Winarni, W. (2018). Teori dan Praktik Penelitian Kuantitatif Kualitatif. Jakarta: Bumi Aksara.
