GROWTH PERFORMANCE AND DIGESTIVE ENZYME ACTIVITY OF NILE TILAPIA FISH (Oreochromis niloticus) FASTED PERIODICALLY IN STAGNANT EARTH POND
Authors
Komar Rudin , Josita Josita , Ricky Djauhari , Uras Tantulo , Ivone ChristianaDOI:
10.29303/jp.v15i5.1822Published:
2025-10-25Issue:
Vol. 15 No. 5 (2025): JURNAL PERIKANANKeywords:
Fasting, Digestive enzyme activity, Growth, Oreochromis niloticusArticles
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Abstract
Nile Tilapia (Oreochromis niloticus) is a freshwater fish species with high economic value and is widely cultivated due to its physiological adaptability and high tolerance to environmental conditions and available feed resources. One problem in aquaculture is suboptimal feed utilization efficiency. A fasting program followed by adequate feeding is a feeding management strategy expected to support growth acceleration and improve feed utilization efficiency. This study aimed to evaluate the effect of one week of fasting on muscle glycogen levels, amylase, protease, and lipase enzyme activities, feed utilization efficiency, feed conversion ratio (FCR), daily growth rate, and survival rate in tilapia (O. niloticus) fry. The data observed included muscle glycogen levels, amylase, protease, and lipase enzyme activities, feed consumption (FGR), feed utilization efficiency (FEE), feed conversion ratio (FCR), daily growth rate (DGR), and survival rate (SGR). This research activity was conducted from April to May 2025, in a family-owned fish pond, Palangka Raya, Central Kalimantan. This study used an experimental method with a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments tested were feeding fasting with treatment A (daily feeding), B (1 day fasting in 1 week), C (2 days fasting in 1 week) and D (3 days fasting in 1 week). The test fish used were tilapia (O. niloticus) seeds with an average weight of 2.01-2.60 g. Feeding was carried out once a day between 08.00 and 12.00 with the amount of feed given per day of 3.75% of the fish biomass weight. The test fish were kept at a stocking density of 30 fish/container. The maintenance container used a hapa net measuring (1x1x1) m3, with a maintenance period of 28 days. The feed used was commercial pellet feed with a protein content of 39%. The results showed that fasting duration significantly (P<0.05) affected muscle glycogen levels and amylase, protease, and lipase enzyme activities, but not significantly (P>0.05) on EP, FCR, LPH, and TKH of tilapia fry. Treatment B (1 day of fasting per week) produced the highest muscle glycogen levels of 140.14 mg/100 mL, the highest EP value of 88.50%, the lowest FCR value of 1.14, the highest LPH value of 2.74%/day, the highest harvested biomass value of 146.63 g, and the highest TKH value of 87.78%.
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