EKSPLORASI AGEN ANTIBAKTERI EKSTRAK SPONS LAUT YANG DIAMBIL DARI PERAIRAN SEKITAR PLTU PAITON PROBOLINGGO

Abstract

This study aims to determine the screening of antibacterial agents and antibacterial activity of marine sponges taken from the waters of PLTU Paiton against the pathogenic bacteria Staphylococcus aureus, Vibrio parahaemolyticus, Escherichia coli and Escherichia coli MDR (Multi Drug Resistant). This study uses an experimental laboratory method. The samples found were 3 sponges and macerated using methanol solvent with 3 repetitions. The results of the sponge extract paste were tested by disc diffusion method against pathogenic bacteria S. aureus, V. parahaemolyticus, E. coli and E. coli MDR. Antibacterial test using a concentration of 10 mg/ml with 2 repetitions. The results showed that PPJT05 produced an inhibition zone of more than 2.4 mm in the four pathogenic bacteria tested. PPJT02 extract produced the highest inhibition zone against V. parahaemolyticus bacteria. The highest antibacterial activity of S. aureus was shown by PPJT05 extract. The highest antibacterial activity of E. coli was shown by PPJT05 extract. The highest MDR E. coli antibacterial activity was shown by PPJT05 extract. PPJT03 extract did not show activity against MDR E. coli bacteria although this extract was active against other bacteria. PPJT02, PPJT03 and PPJT05 extracts have broad-spectrum antibacterial compounds characterized by the inhibitory zone ability against gram-positive and gram-negative bacteria.

Key words: Antibacterial, Sponge, S. aureus, V. parahaemolyticus, PLTU Paiton